This study compares methods to reduce activation signature gene expression in PBMCs, finding that cold isolation is effective and cost-efficient, while TTis impair cellular responsiveness.

bioRxiv · Jul 31, 2023Immunology

Standardized quality control workflow to evaluate the reproducibility and differentiation potential of human iPSCs into neurons

C. X.- Q. Chen, N. Abdian, G. Maussion, R. A. Thomas, I. Demirova, E. Cai, M. Tabatabaei, L. K. Beitel, J. Karamchandani, E. A. Fon, T. M. Durcan

The paper establishes a standardized quality control workflow to assess variability and reproducibility of human iPSCs in differentiation into neurons and other germ layers.

bioRxiv · Jan 13, 2021 · PreprintCell Biology

CASCADES, a novel SOX2 super-enhancer associated long noncoding RNA, regulates cancer stem cell specification and differentiation in glioblastoma multiforme

U. Shahzad, C. Li, M. Johnston, J. J. Wang, N. Sabha, F. S. Varn, A. Riemenschneider, S. Krumholtz, P. Meda, C. Smith, J. Karamchandani, J. K. Watts, R. G. W. Verhaak, M. Gallo, J. T. Rutka, S. Das

This study identifies CASCADES, a lncRNA that regulates stemness in glioblastoma cancer stem cells by epigenetically controlling SOX2 expression.

bioRxiv · Sep 5, 2020 · PreprintCancer Biology