J. Kopka

Positional 13C Enrichment Analysis of Aspartate by GC-MS to Determine PEPC Activity In Vivo

This paper develops a GC-MS-based method to measure PEPC activity in vivo by analyzing positional 13C enrichment in aspartate from Synechocystis cultures.

Remodelled Ribosomes Synthesise a Specific Proteome in Proliferating Plant Tissue during Cold

The paper shows that during cold acclimation in barley root meristems, ribosomal proteins are selectively synthesized and assembled, leading to changes in ribosome composition that may influence translation initiation.

Convergence and molecular evolution of floral fragrance after independent transitions to self fertilization

The paper investigates convergent evolution of floral fragrance in self-fertilizing Capsella species, finding shared compound changes and mutations affecting scent emission.

PEPc-mediated CO2 assimilation provides carbons to gluconeogenesis and the TCA cycle in both dark-exposed and illuminated guard cells

This study uses 13C-labelling to show that PEPc-mediated CO2 assimilation supplies carbons for gluconeogenesis and the TCA cycle in both dark and illuminated guard cells, with illumination increasing 13C-enrichment in several metabolites.

Homologs of circadian clock proteins impact the metabolic switch between light and dark growth in the cyanobacterium Synechocystis sp. PCC 6803

This study shows that mutations in circadian clock protein homologs in Synechocystis affect metabolic changes during light-to-dark transitions, with rpaA having a stronger impact on carbon and nitrogen metabolism.

Distinct metabolic flux modes through the tricarboxylic acid cycle in mesophyll and guard cells revealed by GC-MS-based 13C-positional isotopomer analysis

The paper uses 13C-positional isotopomer analysis to compare metabolic flux modes in mesophyll and guard cells, revealing distinct TCA cycle dynamics and roles of the mitochondrial thioredoxin system.

Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation

This study shows Arabidopsis REIL proteins are essential for ribosome biogenesis during cold acclimation, affecting ribosome composition and suppressing pre-initiation complexes.

The fungal endophyte Fusarium solani provokes differential effects on the energy balance of two Lotus species

This study examines how the fungal endophyte Fusarium solani affects energy balance in two Lotus species, showing differential impacts on growth and metabolic parameters.