BiochemistrybioRxiv
Dual site-specific chemoenzymatic antibody fragment conjugation using CRISPR-based hybridoma engineering
C. M. Le Gall, J. M. van der Schoot, I. Ramos-Tomillero…·M. Verdoes
This paper introduces a CRISPR-based method to engineer hybridoma cells that secrete dual-tagged Fab fragments, enabling site-specific conjugation of multiple cargos.
DOI 10.1021/acs.bioconjchem.0c00673