J. Rozsypal

Insect cell plasma membranes do, while soluble enzymes do not, need stabilization by accumulated cryoprotectant molecules during freezing stress.

The paper shows that freeze-sensitive insects' soluble enzymes remain active during freezing stress, but their plasma membranes lose integrity, which is prevented by cryoprotectants in freeze-tolerant insects.

A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.

The paper identifies and quantifies cryoprotectants in a freeze-tolerant fly larva and shows their synergistic effects in cryopreservation.