Insect cell plasma membranes do, while soluble enzymes do not, need stabilization by accumulated cryoprotectant molecules during freezing stress.
R. Grgac, J. Rozsypal, L. Des Marteaux, T. Stetina, V. Kostal
The paper shows that freeze-sensitive insects' soluble enzymes remain active during freezing stress, but their plasma membranes lose integrity, which is prevented by cryoprotectants in freeze-tolerant insects.
bioRxiv · Jun 23, 2022 · PreprintPhysiology