High-throughput engineering and modification of non-ribosomal peptide synthetases based on Golden Gate assembly
Adrian Podolski, Timon A. Lindeboom, Leonard Präve, Maryam Dehghan, Hannah A. Minas, Janik Kranz, Daniel Schindler, Helge B. Bode
This paper describes a Golden Gate assembly method for high-throughput engineering of non-ribosomal peptide synthetases (NRPS) to generate novel peptides and modify natural products.
Angewandte Chemie International Edition · Oct 11, 2025Peer-reviewedSynthetic Biology