Daniel Schindler

This paper presents a coupled strategy for enzyme discovery, evolution, and yeast chassis adaptation to improve microbial biopolymer valorisation, demonstrating it with laccases for lignin depolymerisation.

The paper studies the evolution of nonribosomal peptides in entomopathogenic bacteria, finding evidence of both neutral drift and natural selection driving structural convergence and toxicity diversification.

This paper describes a Golden Gate assembly method for high-throughput engineering of non-ribosomal peptide synthetases (NRPS) to generate novel peptides and modify natural products.

The paper identifies ligand specificities of chemosensory receptors in the human gut microbiota, revealing key metabolites like lactate and formate as important signals.

The paper investigates the evolutionary dynamics of self-assembly in prokaryotic citrate synthases, finding frequent transitions between different assembly types without strong functional consequences.

The paper evaluates the impact of seed region length on synthetic bacterial small RNAs using a library-based approach and shows 12 nucleotides suffice for regulation with Hfq.

The paper demonstrates the use of L-SCRaMbLE to generate large-scale genome rearrangements in synthetic Sc2.0 yeast chromosomes through light-inducible Cre recombinase.

This study identifies NAD-capped RNAs during T4 phage infection of E. coli, showing dynamic changes in NAD-capping and revealing a phage-encoded Nudix hydrolase involved in NAD-RNA decapping.

The paper introduces In-Cloning and Out-Cloning workflows for scarless DNA assembly using SapI enzyme, enabling codon-based fusion and compatible plasmid construction for model organisms.

The paper constructs a synthetic disomic yeast using SCRaMbLE and a newly synthesized chromosome to study aneuploidy phenotypes, finding that both copy number and specific chromosomal regions affect fitness.

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

This study demonstrates that genomic rearrangements in yeast can be efficiently induced using SCRaMbLE, leading to diverse genetic changes and phenotypic effects.

This paper designs and synthesizes a functional neochromosome in yeast that replaces a native chromosome arm, demonstrating genome plasticity and synthetic chromosome viability.