Engineering a Bright Near-Infrared Fluorescent Protein by Screening a Comprehensive Phenotypic Landscape
Devon L. Kulhanek, Qiyao Wei, Jared Cason Head, Jessica Hellinger, Zachary Jansen, Andrew R. Gilmour, Thomas H. Segall-Shapiro, Jennifer S. Brodbelt, Syed Muhammad Usama, Ross T Thyer
The paper identifies mutations in smURFP that significantly increase in vivo fluorescence in E. coli through deep mutational scanning and biophysical analysis.
Protein Science · May 6, 2026Peer-reviewedSynthetic Biology