Thomas H. Segall-Shapiro

The paper identifies mutations in smURFP that significantly increase in vivo fluorescence in E. coli through deep mutational scanning and biophysical analysis.

This study evolves a T7 RNA polymerase variant with a capping enzyme to enable efficient gene expression in eukaryotes, showing improved performance in yeast and mammalian cells.

The paper identifies SARS-CoV-2 neutralizing antibodies from convalescent patients and describes a novel quaternary binding mechanism using high-throughput methods.

The paper develops 12 optimized small molecule sensors in E. coli to enable independent gene expression control with high induction and low background.