Ross T Thyer

The paper develops a fluorescent reporter, SeARCh, to quantify selenocysteine incorporation and reports a hybrid biosynthetic pathway achieving 90% Sec incorporation.

The paper identifies mutations in smURFP that significantly increase in vivo fluorescence in E. coli through deep mutational scanning and biophysical analysis.

The paper uses Deep Mutational Scanning and Growth-based Quantitative Sequencing to analyze CymR variants and identify amino acids essential for repressor function and mutations that improve ligand sensitivity.

This paper develops a L-DOPA sensing system and improves biosynthesis via directed evolution, achieving high-efficiency incorporation into proteins.

The paper develops a modular genetic toolkit for environmental Actinomycetes, enabling metabolic engineering and complex circuit design in Rhodococcus, Nocardia, and Gordonia.

The paper develops bicistronic translational control elements (BCDs) that provide consistent gene expression across diverse contexts and species.

This paper evolves highly specific and sensitive biosensors for alkaloids using a combined screening and selection approach, enabling improved microbial production of therapeutic plant metabolites.

This paper introduces a 3D convolutional neural network for protein engineering that identifies gain-of-function mutations, validated by experimental improvements in protein stability.