High accuracy fluorescence-guided cryo-focused ion beam milling
Davis D. Perez, Sophia Betzler, Patrick Cleeve, Carmela Louise M. Villegas, Cali Antolini, Sven Klumpe, Jay Schwartz, John M. Mitchels, Shu‐Hsien Sheu, Peter D. Dahlberg, Bridget O Carragher, David A. Agard, Julia Peukes, Garrett A. Greenan
This paper introduces two fluorescence-guided cryo-FIB milling workflows to improve targeting accuracy for cryo-ET, enabling routine capture of structures across spatial scales.
bioRxiv · Jun 25, 2026 · PreprintCell Biology