Shu‐Hsien Sheu

This paper introduces two fluorescence-guided cryo-FIB milling workflows to improve targeting accuracy for cryo-ET, enabling routine capture of structures across spatial scales.

AreTomo3 and DenoisET form an automated GPU pipeline that reconstructs cryo-electron tomograms with tile-based contrast transfer function correction, screens their quality, and denoises them during acquisition.

The paper introduces MOSAIC, a multimodal adaptive optical microscope that enables non-invasive imaging across biological scales in live specimens.

The paper uses spatial proteomics to analyze primary cilia proteomes across cell lines, revealing cell-type specificity and single-cilia heterogeneity, and identifies novel cilia proteins and potential genetic variants.

The paper shows that astrocytes detoxify fatty acids from hyperactive neurons through mitochondrial oxidation, protecting neurons from toxicity.

The paper combines expansion and lattice light sheet microscopy to enable high-speed, nanoscale molecular imaging of neural circuits across large volumes.