Sven Klumpe

High accuracy fluorescence-guided cryo-focused ion beam milling

This paper introduces two fluorescence-guided cryo-FIB milling workflows to improve targeting accuracy for cryo-ET, enabling routine capture of structures across spatial scales.

Exploring shaped focused ion beams for lamella preparation

This paper explores the use of shaped focused ion beams for preparing TEM lamellae, demonstrating the feasibility of cellular lamella milling with an ion knife.

The small GTPase Ran defines Nuclear Pore Complex asymmetry

This study shows that the small GTPase Ran controls the asymmetry of nuclear pore complexes by regulating their peripheral configuration in budding yeast and Drosophila.

Reduction of SEM charging artefacts in native cryogenic biological samples.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography

This study uses cryo-ET to visualize copia retrotransposon structure in Drosophila cells, revealing nuclear clustering and size selection during gametogenesis.

Making plant tissue accessible for cryo-electron tomography

This paper describes optimized cryo-ET sample preparation workflows for plant tissues, achieving subnanometer resolution through targeted FIB milling and lamella trimming.

Optimizing Cryo-FIB Lamellas for sub-5 Angstrom in situ Structural Biology

This paper introduces a method using metallic platinum sputtering to improve subtomogram averages, enabling sub-5 Angstrom resolution of yeast ribosomes with fewer particles.

A streamlined workflow for automated cryo focused ion beam milling

The paper introduces hardware and software improvements to enhance cryo-FIB milling for cryo-electron tomography.