John T. Sauls

Tools and methods for high-throughput single-cell imaging with the mother machine

The paper introduces a new image analysis pipeline for mother machine data and evaluates its performance against existing tools, highlighting parameter sensitivity and segmentation biases.

Mother machine image analysis with MM3

MM3 is a modular image analysis pipeline for mother machine experiments that converts raw images into cell feature data within a day.

Gram-positive and Gram-negative Bacteria Share Common Principles to Coordinate Growth and the Cell Cycle at the Single-cell Level

This study shows Gram-positive and Gram-negative bacteria share common principles in coordinating growth and the cell cycle at the single-cell level, including invariant cell size per replication origin and a shared hierarchy of physiological parameters.

Mechanistic origin of cell-size control and homeostasis in bacteria

This study identifies the mechanistic basis of cell-size homeostasis in bacteria, showing it results from threshold accumulation and balanced biosynthesis, and demonstrates how to reprogram this process.

Invariance of initiation mass and predictability of cell size in Escherichia coli

The paper shows that cell size in Escherichia coli can be predicted by three biosynthesis variables and reveals the invariance of initiation mass under various perturbations.