Steven D. Brown

Tools and methods for high-throughput single-cell imaging with the mother machine

The paper introduces a new image analysis pipeline for mother machine data and evaluates its performance against existing tools, highlighting parameter sensitivity and segmentation biases.

Intestinal Transgene Delivery with Native E. coli Chassis Allows Persistent Physiological Changes

This study uses native E. coli as chassis to deliver transgenes, inducing persistent physiological changes in conventionally-raised hosts.

Cell-free prototyping enables implementation of optimized reverse beta-oxidation pathways in heterotrophic and autotrophic bacteria

The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.

Mother machine image analysis with MM3

MM3 is a modular image analysis pipeline for mother machine experiments that converts raw images into cell feature data within a day.