Fangwei Si

Emergent, cost-free surplus of core biosynthesis governs bacterial fitness

This study quantifies surplus of core biosynthesis components in E. coli and shows it has no cost to steady-state growth, instead aiding adaptation and reducing cell death.

Power-law memory governs bacterial adaptation and learning in fluctuating environments

This study shows bacteria adapt to fluctuating environments using power-law memory, linking cellular dynamics to learning-like behavior through a theoretical model and experimental validation.

Tools and methods for high-throughput single-cell imaging with the mother machine

The paper introduces a new image analysis pipeline for mother machine data and evaluates its performance against existing tools, highlighting parameter sensitivity and segmentation biases.

Quantitative examination of five stochastic cell-cycle and cell-size control models for Escherichia coli and Bacillus subtilis

The paper evaluates five models of cell-cycle and cell-size control in bacteria, finding the Independent Double Adder model most consistent with experimental data.

Single-cell data and correlation analysis support the independent double adder model in both Escherichia coli and Bacillus subtilis

The paper challenges the RDA model by showing it cannot compare biological models and is incompatible with the adder principle, supporting the IDA model instead.

Comment on 'Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism'

The paper reanalyzes Witz et al.'s data and performs additional experiments, supporting the division-centric model of cell-size control in E. coli.

Mother machine image analysis with MM3

MM3 is a modular image analysis pipeline for mother machine experiments that converts raw images into cell feature data within a day.

Mechanistic origin of cell-size control and homeostasis in bacteria

This study identifies the mechanistic basis of cell-size homeostasis in bacteria, showing it results from threshold accumulation and balanced biosynthesis, and demonstrates how to reprogram this process.

Invariance of initiation mass and predictability of cell size in Escherichia coli

The paper shows that cell size in Escherichia coli can be predicted by three biosynthesis variables and reveals the invariance of initiation mass under various perturbations.