An in-cell approach to evaluate E3 ligases for use in targeted protein degradation.
Yunan Zheng, Anamika Singh, Zeqi Niu, Violeta L. Marin, Jonathon Young, Paul L. Richardson, Marcus L. Hemshorn, Richard B. Cooley, P. Andrew Karplus, Kedar Puvar, Scott E. Warder, Anil Vasudevan, Justin M. Reitsma, Ryan A. Mehl
This paper introduces an in-cell method to evaluate E3 ligases for targeted protein degradation by conjugating neo-substrates via click chemistry.
Journal of the American Chemical Society · Jun 10, 2025Peer-reviewedCell Biology