Methylation pseudotime analysis for label-free profiling of the temporal chromatin landscape with long-read sequencing
Annie Trinh, Navied Akhtar, Kwadwo Bonsu, Nandor Laszik, Asia Mendelevich, Tanye Wen, Julien L. P. Morival, Katelyn E. Diune, Mitchell Frazeur, Justin E. Vega, Alexander A. Gimelbrant, Elizabeth L. Read, Timothy L. Downing
This paper introduces MPATH, a method to temporally order epigenetic modifications using long-read sequencing, revealing sub-cell-cycle dynamics of methylation states and nucleosome occupancy.
bioRxiv · Mar 3, 2025 · PreprintGenomics