PAM recognition by miniature CRISPR nucleases triggersprogrammable double-stranded DNA target cleavage
T. Karvelis, G. Bigelyte, J. K. Young, Z. Hou, R. Zedaveinyte, K. Budre, S. Paulraj, V. Djukanovic, S. Gasior, A. Silanskas, C. Venclovas, V. Siksnys
This study identifies and characterizes miniature CRISPR-Cas nucleases that cleave double-stranded DNA in a PAM-dependent manner, showing their potential for programmable genome editing.
bioRxiv · Feb 20, 2020Biochemistry