Author

T. Karvelis

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Biophysics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Assay:DNA methylation· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1 Assay:imaging· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

V. Siksnys· 3 shared C. Venclovas· 2 shared G. Bigelyte· 2 shared G. Gasiunas· 2 shared J. K. Young· 2 shared S. Paulraj· 2 shared Z. Hou· 2 shared A. Kopustas· 1 shared

Activity by Year

2020-2020 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

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3 shared papersBiochemistry

V. Siksnys

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Oriented Soft DNA Curtains for Single Molecule Imaging

A. Kopustas, S. Ivanovaite, T. Rakickas, E. Poceviciute, J. Paksaite, T. Karvelis, M. Zaremba, E. Manakova, M. Tutkus

This paper introduces a new method for creating oriented DNA curtains using protein templates to study protein-DNA interactions with improved stability and controllability.

bioRxiv · Jun 15, 2020Biophysics

Biochemically diverse CRISPR-Cas9 orthologs

G. Gasiunas, J. K. Young, T. Karvelis, D. Kazlauskas, T. Urbaitis, M. Jasnauskaite, M. Grusyte, S. Paulraj, P.-H. Wang, Z. Hou, S. K. Dooley, M. Cigan, C. Alarcon, N. D. Chilcoat, G. Bigelyte, , V. Siksnys

The paper characterizes the biochemical diversity of CRISPR-Cas9 orthologs, identifying varied PAM recognition and gRNA requirements across 79 Cas9 proteins.

bioRxiv · Apr 29, 2020 · PreprintBiochemistry

5' modifications to CRISPR Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

G. Mullally, K. van Aelst, M. M. Naqvi, F. M. Diffin, T. Karvelis, G. Gasiunas, V. Siksnys, M. D. Szczelkun

The paper investigates how 5' modifications to CRISPR gRNA affect R-loop dynamics and DNA cleavage, finding that certain modifications inhibit cleavage while others do not.

bioRxiv · Apr 9, 2020Biochemistry

PAM recognition by miniature CRISPR nucleases triggersprogrammable double-stranded DNA target cleavage

T. Karvelis, G. Bigelyte, J. K. Young, Z. Hou, R. Zedaveinyte, K. Budre, S. Paulraj, V. Djukanovic, S. Gasior, A. Silanskas, C. Venclovas, V. Siksnys

This study identifies and characterizes miniature CRISPR-Cas nucleases that cleave double-stranded DNA in a PAM-dependent manner, showing their potential for programmable genome editing.

bioRxiv · Feb 20, 2020Biochemistry