Illuminating chromatin compaction in live cells and fixed tissues using SiR-DNA fluorescence lifetime
C. Hockings, C. Poudel, K. A. Feeney, C. L. Novo, M. S. Hamouda, I. Mela, D. Fernandez-Antoran, P. P. Vallejo Ramirez, P. Rugg-Gunn, K. Chalut, C. F. Kaminski, G. S. Kaminski Schierle
This paper develops a method to measure chromatin compaction in live and fixed cells using SiR-DNA fluorescence lifetime, revealing compaction in differentiating cells and decompaction during stem cell transitions.
bioRxiv · May 2, 2020 · PreprintCell Biology