Author

K. K. Brower

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 2 Research Focus:cell type annotation· 2 Research Focus:clustering· 2 Assay:flow cytometry· 2 Assay:bulk RNA-seq· 1 Research Focus:cell-cell communication· 1 Organism:human· 1

Frequent Co-Authors

P. M. Fordyce· 4 shared G. Kim· 3 shared B. Wang· 2 shared M. Khariton· 2 shared S. G. K. Calhoun· 2 shared S. Klemm· 2 shared A. L. McCully· 1 shared B. Cruz· 1 shared

Activity by Year

2019-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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4 shared papersBioengineering

P. M. Fordyce

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4 papers

Recent·Most cited

Alleviating cell lysate-induced inhibition to enable RT-PCR from single cells in picoliter-volume double emulsion droplets

M. Khariton, C. McClune, K. K. Brower, S. Klemm, E. Sattely, P. M. Fordyce, B. Wang

This study addresses RT-PCR inhibition in picoliter double emulsion droplets by identifying lysate-induced probe cleavage as the main issue and showing heat lysis can alleviate it, enabling single-cell PCR in 20 pL droplets.

bioRxiv · Aug 16, 2022Bioengineering

Systematic Characterization of Double Emulsion Droplets for Biological Applications

S. G. K. Calhoun, K. K. Brower, V. Chandran Suja, G. Kim, N. Wang, A. L. McCully, H. Kusumaatmaja, G. G. Fuller, P. M. Fordyce

This paper systematically characterizes double emulsion droplets to predict their size and stability based on flow rates and buffer properties, enabling better design of bioassays.

bioRxiv · Mar 4, 2022 · PreprintBioengineering

Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

K. K. Brower, M. Khariton, P. H. Suzuki, C. Still, G. Kim, S. G. Calhoun, L. S. Qi, B. Wang, P. M. Fordyce

The paper introduces Dropception, a method for high-throughput single-cell encapsulation and phenotyping using double emulsion picoreactors and FACS.

bioRxiv · Jun 7, 2020Bioengineering

Optimized double emulsion flow cytometry with high-throughput single droplet isolation

K. K. Brower, C. Carswell-Crumpton, S. Klemm, B. Cruz, G. Kim, S. G. K. Calhoun, L. Nichols, P. M. Fordyce

This paper introduces sdDE-FACS, a method for high-throughput phenotyping and sorting of double emulsion droplets using flow cytometry, achieving >70% single droplet recovery and efficient nucleic acid extraction.

bioRxiv · Nov 8, 2019Bioengineering