P. M. Fordyce

FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments

This paper develops FACS-sortable triple emulsion picoreactors for high-throughput screening of biphasic reactions, demonstrating stability and compatibility with protein synthesis.

High-throughput thermodynamic and kinetic measurements of transcription factor/DNA mutations reveal how conformational heterogeneity can shape motif selectivity

The paper measures thermodynamic and kinetic properties of transcription factor/DNA mutations to show how conformational heterogeneity affects motif selectivity.

Design Automation of Microfluidic Single and Double Emulsion Droplets with Machine Learning

This paper trains machine learning models to predict microfluidic device geometries and flow conditions for generating stable emulsions, achieving high accuracy and creating a design automation tool.

Global substrate identification and high throughput in vitro dephosphorylation reactions uncover PP1 and PP2A-B55 specificity principles

The paper develops an in vitro assay to determine phosphatase specificity and applies it to study mitotic exit, revealing substrate preferences and biological relevance.

High-throughput enzymology reveals mutations throughout a phosphatase that decouple catalysis and transition state analog affinity

This study uses HT-MEK to analyze 1,004 PafA mutants, showing that distal mutations affect catalysis more than TSA binding, suggesting conformational changes influence enzyme function.

A microwell platform for high-throughput longitudinal phenotyping and selective retrieval of organoids

This paper introduces a microwell platform for high-throughput longitudinal phenotyping of organoids and their selective retrieval for molecular profiling, identifying genomic and expression changes linked to growth and polarity.

Double emulsions as a high-throughput enrichment and isolation platform for slower-growing microbes

This study introduces GrowMiDE, a high-throughput platform for enriching and isolating slower-growing microbes by encapsulating them in double emulsions, demonstrating its effectiveness in cultivating novel taxa and preventing competition from fast-growing species.

Alleviating cell lysate-induced inhibition to enable RT-PCR from single cells in picoliter-volume double emulsion droplets

This study addresses RT-PCR inhibition in picoliter double emulsion droplets by identifying lysate-induced probe cleavage as the main issue and showing heat lysis can alleviate it, enabling single-cell PCR in 20 pL droplets.

Short tandem repeats bind transcription factors to tune eukaryotic gene expression

This study shows STRs modulate TF-DNA interactions by altering binding affinities and on rates, impacting gene expression through increased TF density near target sites.

Systematic Characterization of Double Emulsion Droplets for Biological Applications

This paper systematically characterizes double emulsion droplets to predict their size and stability based on flow rates and buffer properties, enabling better design of bioassays.

MRBLE-pep measurements reveal accurate binding affinities for B56, a PP2A regulatory subunit

The paper compares MRBLE-pep and ITC measurements of B56 binding affinities, showing MRBLE-pep accurately quantifies relative affinity with less material.

Structure-activity mapping of the force- and sequence-dependent landscape of T cell activation

This paper introduces BATTLES, a high-throughput method that applies biomechanical force to screen pMHCs for T-cell activation, revealing force- and sequence-dependent responses.

Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

HT-MEK, a microfluidic platform, reveals enzyme architectures shaping function through high-throughput kinetic analysis of enzyme variants.

MRBLEs 2.0: High-throughput generation of chemically functionalized spectrally and magnetically-encoded hydrogel beads using a simple single-layer microfluidic device

The paper introduces MRBLEs 2.0, a method for high-throughput synthesis of functionalized microspheres with spectral and magnetic encoding, demonstrating efficient conjugation of biomolecules and magnetic nanoparticle incorporation.

High-throughput binding affinity measurements for mutations spanning a transcription factor-DNA interface reveal affinity and specificity determinants

The paper measures binding affinities of TF mutants and DNA variants to identify affinity and specificity determinants in the TF-DNA interface.

Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

The paper introduces Dropception, a method for high-throughput single-cell encapsulation and phenotyping using double emulsion picoreactors and FACS.

Optimized double emulsion flow cytometry with high-throughput single droplet isolation

This paper introduces sdDE-FACS, a method for high-throughput phenotyping and sorting of double emulsion droplets using flow cytometry, achieving >70% single droplet recovery and efficient nucleic acid extraction.

micrIO: An Open-Source Autosampler and Fraction Collector for Automated Microfluidic Input-Output

The paper introduces micrIO, an open-source platform for automated microfluidic input-output, demonstrating its ability to handle samples with no cross-contamination.

Live imaging of Aiptasia larvae, a model system for studying coral bleaching, using a simple microfluidic device

This paper introduces a microfluidic device to image live Aiptasia larvae and their algal symbionts under environmental stress, observing algal expulsion as a potential mechanism of coral bleaching.

Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads

This study introduces MRBLE-pep, a method to quantify protein-peptide affinity landscapes, revealing critical factors influencing binding and identifying potential therapeutic peptides.

An Open-Source, Programmable Pneumatic Setup for Operation and Automated Control of Single- and Multi-Layer Microfluidic Devices

The paper introduces an open-source, programmable pneumatic setup for operating single- and multi-layer microfluidic devices with automation and cost-effectiveness.