Author

L. S. Qi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 6 Cancer Biology· 2 Cell Biology· 2 Microbiology· 2 Immunology· 1

Signature Assays, Organisms & Methods

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Organism:human· 9 Approach:experimental assay· 8 Research Focus:cell type annotation· 6 Research Focus:drug response / drug discovery· 4 Research Focus:host-pathogen & immune response· 4 Assay:CRISPR screen· 2 Assay:fluorescence / live-cell microscopy· 2 Approach:genetic perturbation / CRISPR· 2

Frequent Co-Authors

L. Zeng· 5 shared D. Zhao· 3 shared M. La Russa· 3 shared Y. Liu· 3 shared A. R. Roy· 2 shared H. Wang· 2 shared M. Han· 2 shared W. E. Moerner· 2 shared

Activity by Year

2018-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 22 mutual co-author links

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5 shared papersBioengineering

L. Zeng

Mutual co-authors in cluster

Author profile →

13 papers

Recent·Most cited

Virus-derived Synthetic Riboswitches for Real-time Coronaviruses Sensing in Live Cells

L. Zeng, L. S. Qi

This paper develops a viral riboswitch system for real-time coronavirus detection and antiviral response in live cells, showing effectiveness against 229E and OC43.

bioRxiv · Feb 27, 2024Bioengineering

Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles

L. Andronov, M. Han, Y. Zhu, A. Balaji, A. R. Roy, A. E. S. Barentine, P. Patel, J. Garhyan, L. S. Qi, W. E. Moerner

This study uses super-resolution microscopy to reveal the nanoscale organization of SARS-CoV-2 replication organelles, showing distinct vgRNA and dsRNA clusters and changes over infection stages.

bioRxiv · Nov 7, 2023Microbiology

Biodesign of Frugal CRISPR Kits for Equitable and Accessible Education

M. C. Collins, M. B. Lau, W. Ma, A. Shen, M. La Russa, L. S. Qi

This study develops a frugal CRISPR kit for K-12 education, enabling hands-on genome engineering experiments without traditional lab equipment or high costs.

bioRxiv · Oct 14, 2023Bioengineering

casmini-tool: a comprehensive database for efficient and specific guide RNA design using dCasMINI

S. Lopp, T. Borrman, N. Jha, M. Silvis, R. Swan, Y. Liu, X. Yang, G. Alvarez, C. Klappenbach, D. O. Hart, L. S. Qi, T. P. Daley, R. W. Yeo

The paper introduces casmini-tool, a database for designing dCasMINI guide RNAs with off-target prediction and PAM profile analysis for CRISPRi/a applications.

bioRxiv · Sep 17, 2023 · PreprintBioengineering

SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D

M. J. Lee, M. W. Leong, A. Rustagi, A. Beck, L. Zeng, S. Holmes, L. S. Qi, C. A. Blish

This study shows SARS-CoV-2 evades NK cell killing by downregulating NKG2D ligands via Nsp1, reducing NK cell cytotoxicity against infected cells.

bioRxiv · Jun 20, 2022Immunology

Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing

D. Henn, D. Zhao, K. Chen, A. Trotsyuk, C. A. Bonham, K. S. Fischer, T. Kehl, T. Fehlmann, D. Sivaraj, A. H. Greco, S. E. Moortgat Illouz, J. Padmanabhan, J. A. Barrera, U. Kneser, H.-P. Lenhof, , G. C. Gurtner

This study uses CRISPR/Cas9 to knockout Ndrg2 in dendritic cells, enhancing their regenerative capacity for wound healing in mouse models.

bioRxiv · Mar 14, 2022Cell Biology

Targeted Reactivation of X-linked Endogenous FOXP3 Gene from X-chromosome Inactivation in Human Female Breast Cancer Cells

X. Cui, Z. Xu, S. Wang, X. Li, E. Stringer-Reasor, S. Bea, L. Zeng, D. Zhao, R. Liu, L. S. Qi, L. Wang

This paper develops a CRISPRi/a approach to reactivate FOXP3 and silence XIST from XCI in human breast cancer cells, showing enhanced epigenetic changes and therapeutic potential.

bioRxiv · Aug 14, 2021Cancer Biology

Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection

J. Wang, M. Han, A. R. Roy, H. Wang, L. Moeckl, L. Zeng, W. E. Moerner, L. S. Qi

This study uses multi-color super-resolution imaging to analyze the spatial organization of coronavirus RNA during infection and assess antiviral drug effects.

bioRxiv · Jun 9, 2021Cell Biology

Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

K. K. Brower, M. Khariton, P. H. Suzuki, C. Still, G. Kim, S. G. Calhoun, L. S. Qi, B. Wang, P. M. Fordyce

The paper introduces Dropception, a method for high-throughput single-cell encapsulation and phenotyping using double emulsion picoreactors and FACS.

bioRxiv · Jun 7, 2020Bioengineering

Development of CRISPR as a prophylactic strategy to combat novel coronavirus and influenza

T. R. Abbott, G. Dhamdhere, Y. Liu, X. Lin, L. E. Goudy, L. Zeng, A. Chemparathy, S. Chmura, N. Heaton, R. Debs, T. Pande, D. Endy, M. La Russa, D. B. Lewis, L. S. Qi

This paper develops a CRISPR-Cas13-based strategy called PAC-MAN to inhibit SARS-CoV-2 and influenza A virus in human cells, showing it can degrade viral sequences and reduce replication.

bioRxiv · Mar 13, 2020 · PreprintBioengineering

Systematic genome-wide querying of coding and non-coding functional elements in E. coli using CRISPRi

H. S. Rishi, E. Toro, H. Liu, X. Wang, L. S. Qi, A. P. Arkin

This study uses CRISPRi to systematically analyze coding and non-coding elements in E. coli, revealing conditional essentiality and novel insights into promoter and TFBS targeting.

bioRxiv · Mar 4, 2020 · PreprintMicrobiology

Temporal-Spatial Visualization of Endogenous Chromosome Rearrangements in Living Cells

H. Wang, M. Nakamura, D. Zhao, C. M. Nguyen, C. Yu, A. Lo, T. Daley, M. La Russa, Y. Liu, L. S. Qi

This paper introduces LiveFISH, a method to visualize real-time chromosomal rearrangements in living cells, and demonstrates its application in detecting gene-editing induced translocations.

bioRxiv · Aug 14, 2019 · PreprintBioengineering

YAP-independent mechanotransduction drives breast cancer progression

J. Y. Lee, J. Chang, A. A. Dominguez, S. Nam, J. Chang, H.-p. Lee, S. Varma, L. S. Qi, R. B. West, O. Chaudhuri

This study shows that mechanotransduction in breast cancer occurs without YAP, due to compact nuclear morphologies in vivo.

bioRxiv · Dec 13, 2018Cancer Biology