Author

S. Klemm

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Plant Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:Arabidopsis· 1 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Research Focus:cell-cell communication· 1 Research Focus:clustering· 1 Research Focus:development & morphogenesis· 1

Frequent Co-Authors

K. K. Brower· 2 shared P. M. Fordyce· 2 shared B. Cruz· 1 shared B. Moeller· 1 shared B. Wang· 1 shared C. Carswell-Crumpton· 1 shared C. McClune· 1 shared D. Mitra· 1 shared

Activity by Year

2018-2022 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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2 shared papersBioengineering

K. K. Brower

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3 papers

Recent·Most cited

Alleviating cell lysate-induced inhibition to enable RT-PCR from single cells in picoliter-volume double emulsion droplets

M. Khariton, C. McClune, K. K. Brower, S. Klemm, E. Sattely, P. M. Fordyce, B. Wang

This study addresses RT-PCR inhibition in picoliter double emulsion droplets by identifying lysate-induced probe cleavage as the main issue and showing heat lysis can alleviate it, enabling single-cell PCR in 20 pL droplets.

bioRxiv · Aug 16, 2022Bioengineering

Optimized double emulsion flow cytometry with high-throughput single droplet isolation

K. K. Brower, C. Carswell-Crumpton, S. Klemm, B. Cruz, G. Kim, S. G. K. Calhoun, L. Nichols, P. M. Fordyce

This paper introduces sdDE-FACS, a method for high-throughput phenotyping and sorting of double emulsion droplets using flow cytometry, achieving >70% single droplet recovery and efficient nucleic acid extraction.

bioRxiv · Nov 8, 2019Bioengineering

Microtubule-associated protein IQ67 DOMAIN5 regulates interdigitation of leaf pavement cells in Arabidopsis thaliana

D. Mitra, P. Kumari, J. Quegwer, S. Klemm, B. Moeller, Y. Poeschl, P. Pflug, G. Stamm, S. Abel, K. Bürstenbinder

This study identifies IQD5 as a microtubule-associated protein regulating pavement cell shape in Arabidopsis by linking calcium signaling to interdigitation and asymmetric expansion.

bioRxiv · Feb 20, 2018Plant Biology