Tagging allows faithful tracing of expression and enhances biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.
S. Soliman, A. E. Stark, M. Gardner, S. W. Harshman, C. C. Breece, F. Amari, A. Orlacchio, M. Chen, A. Tessari, J. A. Martin, R. Visone, M. A. Freitas, K. La Perle, D. Palmieri, V. COPPOLA
The paper generates a novel mouse strain with a tagged RanBP9 protein, enabling reliable detection and revealing its interaction with Nucleolin.
bioRxiv · Dec 3, 2019Bioengineering