K. Stachowski

Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM

This study investigates nearest-neighbor cooperativity in TRAP by engineering dTRAP variants and using cryo-EM and thermodynamic analyses to reveal structural and thermodynamic mechanisms of ligand binding cooperativity.

Merlin Tumor Suppressor Function is Regulated by PIP2-Mediated Dimerization

This study shows Merlin dimerization is regulated by PIP2-mediated conformational changes and phosphorylation, with implications for its tumor suppressor function.

Unloxing the assembly and activation mechanism of Cre recombinase using Cryo-EM

This paper determines cryo-EM structures of Cre-loxP assembly intermediates to elucidate mechanisms of site selection, strand cleavage order, and activation in the Cre recombinase system.

DNA Binding Induces a cis to trans Switch in Cre Recombinase to Enable Intasome Assembly

This paper uses NMR to show that Cre recombinase undergoes a cis-to-trans conformational switch upon DNA binding, enabling intasome assembly and DNA recombination.