Personalized CRISPR Knock-In Cytokine Gene Therapy to Remodel the Tumor Microenvironment and Enhance CAR T Cell Therapy in Solid Tumors
Michael Launspach, Julia Macos, Shoaib Afzal, Janik Hohmann, Marc L Appis, Maximilian Pilgram, Stefanie Beez, Emily Ohlendorf, Casper F. T. van der Ven, Chahrazad Lachiheb, Karin Toews, Lena Andersch, Marvin Jens, Felix Zirngibl, Jonas Kath, , Annette Künkele
This paper develops CancerPAM, a bioinformatics pipeline for identifying CRISPR knock-in sites to remodel the tumor microenvironment, enhancing CAR T cell therapy in solid tumors.
Nature Communications · Dec 9, 2025Peer-reviewedBioengineering