Dimitrios L. Wagner

CRISPR-dPCR: A unified multiplex digital PCR framework for quantitative characterization of targeted knock-in

CRISPR-dPCR quantifies CRISPR knock-in by distinguishing integration from donor abundance and background, using multiplex digital PCR with orthogonal strategies.

A Non-Viral CRISPR/Cas9 HDR Platform for Stable Engineering of Solid Tumor Models.

This paper develops a non-viral CRISPR/Cas9 HDR platform for stable transgene knock-in in solid tumor models, achieving up to 60% efficiency in neuroblastoma cells.

Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

Next-generation all-in-one CRISPR/Cas9 multiply-edited CD30CAR-T cells:Potency despite risk of translocations

This study develops next-generation CD30CAR-T cells using CRISPR/Cas9 for multiplex editing, showing high potency but risks of translocations with combined knockouts.

Revisiting the need for mRNA nucleoside modification in CAR T cell engineering

This study shows that unmodified mRNA can be used for CAR-T cell engineering without compromising function, eliminating the need for costly nucleoside modifications.

Personalized CRISPR Knock-In Cytokine Gene Therapy to Remodel the Tumor Microenvironment and Enhance CAR T Cell Therapy in Solid Tumors

This paper develops CancerPAM, a bioinformatics pipeline for identifying CRISPR knock-in sites to remodel the tumor microenvironment, enhancing CAR T cell therapy in solid tumors.

Repurposing base editors for targeted knock-in and simultaneous knockouts to generate multiplex-edited allogeneic CAR T cells with minimal translocations

This paper develops BEKI, a base editor-based method for targeted transgene insertion and multiplex gene disruption in T cells, reducing chromosomal translocations compared to Cas9-based approaches.

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases

This paper introduces one-pot PASTA, a non-viral method for efficient large transgene integration in human T cells using CRISPR and integrases, showing improved performance over existing methods.

Single-stranded HDR templates with truncated Cas12a binding sequences improve knock-in efficiencies in primary human T cells

This study improves CRISPR-Cas12a knock-in efficiency in human T cells by using single-stranded DNA templates with double-stranded Cas-target-sequences, achieving up to 90% knock-in rates for transgene inserts.