Systematic DNA nicking reveals the structural logic of protein recognition
Yumi Minyi Yao, Michael O’Hagan, Karn Onoon, Lihee Givon, Shelly Hamer-Rogotner, Raul Salinas, Naama Kessler, Orly Dym, Tanadet Pipatpolkai, Maria A. Schumacher, Ariel Afek
This study introduces PIC-NIC, a method to systematically perturb DNA binding sites without altering base sequences, revealing how DNA backbone structure influences transcription factor binding.
bioRxiv · Jun 30, 2025 · PreprintBiophysics