Covalent linkage of the DNA repair template to the CRISPR/Cas9 complex enhances homology-directed repair
Nataša Savić, Femke C. A. S. Ringnalda, Helen A. Lindsay, Christian Berk, Katja Bargsten, Yizhou Li, Dario Neri, Mark D. Robinson, Constance Ciaudo, Jonathan Hall, Martin Jínek, Gerald Schwank
This study shows that covalently linking a DNA repair template to the CRISPR/Cas9 complex increases HDR efficiency 23-fold compared to NHEJ.
eLife · May 29, 2018Peer-reviewedMolecular Biology