Martin Jínek

Structural framework for the assembly of the human tRNA ligase complex

This study determines the atomic structure of the human tRNA-ligase complex and reveals its assembly mechanism and functional implications.

Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases

This study investigates the mechanism of transposon excision by type I-F CRISPR-associated transposases using structural and biochemical analyses.

Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases

This study uses cryo-EM to reveal the structural mechanism of RNA-guided DNA integration by PseCAST, providing insights for engineering DNA insertion systems.

Processing of forked DNA activates a helicase-nuclease immune system

The paper shows that forked DNA activates a helicase-nuclease immune complex in type III Druantia through enzymatic processing, enabling phage defense without host toxicity.

Kinetic logic of uridylation-mediated RNA decay

This study investigates how uridylation mediates RNA decay in Drosophila by showing how Tailor generates oligo(U) intermediates and how Dis3l2 decodes these to initiate decay.

Ashwin and FAM98 paralogs define nuclear and cytoplasmic RNA ligase complexes for tRNA biogenesis and the unfolded protein response

This paper identifies Ashwin as a nuclear import factor for the tRNA ligase complex and shows that FAM98 paralogs determine its subcellular localization for tRNA biogenesis and UPR.

Engineering a novel probiotic toolkit in Escherichia coli Nissle1917 for sensing and mitigating gut inflammatory diseases

This paper develops a modular probiotic system in Escherichia coli Nissle1917 that detects nitric oxide and secretes anti-TNF nanobodies to mitigate gut inflammation.

Molecular mechanism of plasmid elimination by the DdmDE defense system

This study uses cryo-EM to elucidate the mechanism of plasmid elimination by the DdmDE defense system in Vibrio cholerae.

DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute

This study characterizes CbAgo, a mesophilic prokaryotic Argonaute that enables DNA-guided DNA cleavage at moderate temperatures using specific siDNA guides.

Covalent linkage of the DNA repair template to the CRISPR/Cas9 complex enhances homology-directed repair

This study shows that covalently linking a DNA repair template to the CRISPR/Cas9 complex increases HDR efficiency 23-fold compared to NHEJ.