Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst
Yong Hyun Kwon, Ljubica Mihaljević, Keehun Kim, David E. Kim, Thomas Connor Donahue, Erik J. Bidstrup, Chandra Kanth Bandi, Belen Sotomayor, Sophia W. Hulbert, Kathryn A. A. Myers, Anru Tian, Mariah Culpepper, Dario Mizrachi, Thapakorn Jaroentomeechai, Henrik Clausen, , Matthew P. DeLisa
The paper describes methods to make a membrane-bound glycosyltransferase water-soluble for easier study and application in synthetic glycobiology.
bioRxiv · Jan 31, 2026 · PreprintSynthetic Biology