Sophia W. Hulbert

Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst

The paper describes methods to make a membrane-bound glycosyltransferase water-soluble for easier study and application in synthetic glycobiology.

A scalable cell-free manufacturing platform for two-step bioproduction of immunogenic conjugate vaccines

This paper presents a cell-free manufacturing platform for producing conjugate vaccines with high glycosylation efficiency and purity, validated through immunogenicity testing in mice.

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in Escherichia coli

The paper identifies a single-subunit oligosaccharyltransferase from Desulfovibrio marinus that enables glycosylation of full-length IgG antibodies in E. coli.

Shotgun scanning glycomutagenesis: a simple and efficient strategy for constructing and characterizing neoglycoproteins

This paper introduces a method to construct and analyze neoglycoproteins by systematically modifying glycosylation sites in proteins and assessing the effects of glycan addition.