Thomas Connor Donahue

PepRePs: Peptide-Retargeted Phosphatases Designed with Generative Language Models

This paper introduces PepRePs, a system using peptide-retargeted phosphatases to selectively manipulate protein phosphorylation, showing reduced phosphorylation and downstream effects on cellular processes.

Programmable protein degraders enable selective knockdown of pathogenic β-catenin subpopulations in vitro and in vivo

This paper develops programmable protein degraders (uAbs) that selectively knock down pathogenic β-catenin in vitro and in vivo, reducing tumor cell survival and proliferation.

Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst

The paper describes methods to make a membrane-bound glycosyltransferase water-soluble for easier study and application in synthetic glycobiology.

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in Escherichia coli

The paper identifies a single-subunit oligosaccharyltransferase from Desulfovibrio marinus that enables glycosylation of full-length IgG antibodies in E. coli.