Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria
Daphne Collias, Elena Vialetto, Jiaqi Yu, Khoa Co, Éva d. H. Almási, Ann-Sophie Rüttiger, Tatjana Achmedov, Till Strowig, Chase L. Beisel
This paper shows that reducing DNA targeting activity in CRISPR systems improves genome editing efficiency in bacteria by enabling RecA-mediated repair.
Nature Communications · Feb 8, 2023Peer-reviewedSynthetic Biology