Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase
Edward Kalkreuter, Kyle S. Bingham, Aaron M. Keeler, Andrew N. Lowell, Jennifer J. Schmidt, David H. Sherman, Gavin J. Williams
The paper identifies key residues in an acyltransferase that influence substrate selectivity and demonstrates how swapping structural motifs can alter substrate specificity in polyketide synthases.
Nature Communications · Apr 13, 2021Peer-reviewedSynthetic Biology