Fragmentation of macrophages during isolation confounds analysis of single cell preparations from mouse hematopoietic tissues
S. M. Millard, O. Heng, K. S. Opperman, A. Sehgal, K. M. Irvine, S. Kaur, K. M. Summers, C. J. Sandrock, A. C. Wu, G. W. Magor, L. Batoon, A. C. Perkins, J. E. Noll, A. C. Zannettino, D. P. Sester, , A. R. Pettit
The paper identifies macrophage fragmentation during isolation as a confounding factor in single-cell analysis of mouse hematopoietic tissues, revealing its impact on marker expression and function.
bioRxiv · Apr 28, 2021Immunology