Dual site-specific chemoenzymatic antibody fragment conjugation using CRISPR-based hybridoma engineering
C. M. Le Gall, J. M. van der Schoot, I. Ramos-Tomillero, M. Parlak Khalily, F. J. van Dalen, Z. Wijfjes, L. Smeding, D. van Dalen, A. Cammarata, K. M. Bonger, C. G. Figdor, F. A. Scheeren, M. Verdoes
This paper introduces a CRISPR-based method to engineer hybridoma cells that secrete dual-tagged Fab fragments, enabling site-specific conjugation of multiple cargos.
bioRxiv · Dec 8, 2020Biochemistry