Evolution of CRISPR-associated Endonucleases as Inferred from Resurrected Proteins
B. Alonso-Lerma, Y. Jabalera, M. Morin, A. Fernandez, S. Samperio, A. Quesada-Ganuza, A. Reifs, S. Fernandez-Penalver, Y. Benitez, L. Soletto, J. A. Gavira, A. Diaz, W. Vranken, B. P. Kleinstiver, A. Sanchez-Mejias, , R. Perez-Jimenez
This study resurrects ancient CRISPR-Cas9 nucleases to show their greater flexibility in PAM and gRNA requirements and their evolution toward double-strand break activity.
bioRxiv · Mar 30, 2022Evolutionary Biology