A. Fernandez

Free Amino Acids Accelerate the Time-Dependent Inactivation of Rat Liver Nucleotide Pyrophosphatase / Phosphodiesterase Enpp3 elicited by EDTA

The paper investigates how free amino acids affect the EDTA-induced inactivation of rat liver NPP/PDE Enpp3 and identifies Enpp3 as a 2,3-cGAMP hydrolase.

AI-assisted decoding of molecular signatures essential for NON-PHOTOTROPIC HYPOCOTYL3 condensation and function in phototropism

The paper uses AI-based protein structure prediction to identify a bipartite motif in NPH3 essential for its condensation and function in phototropism.

Directed evolution for cell separation in natural isolates of budding yeast reveals selection to deactivate AMN1 and the Rim101 pathway in haploids and selection in favor of Hawthorne's deletion in diploids

The paper evolves natural yeast isolates to select for cell separation, identifying AMN1 deactivation and Rim101 pathway mutations in haploids, and Hawthorne's deletion in diploids as key adaptations.

A single-cell atlas of the murine pancreatic ductal tree identifies novel cell populations with potential implications in pancreas regeneration and exocrine pathogenesis.

This study identifies 15 novel ductal cell populations in the mouse pancreas using scRNA-seq and investigates their roles in regeneration and exocrine pathogenesis.

Vitamin D deficiency induces erectile dysfunction: role of superoxide

This study shows vitamin D deficiency causes erectile dysfunction by increasing superoxide production in penile tissue.

De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8

This paper designs highly selective miniprotein inhibitors for integrins αvβ6 and αvβ8 with picomolar affinity and therapeutic potential in a mouse fibrosis model.

Modulation of functional phosphorylation sites by basic residues in the Unique domain of c-Src

The paper investigates how basic residues modulate phosphorylation sites in the SNRE of c-Src using NMR and mutations, revealing electrostatic interactions and their effects on conformation.

Immunogenicity and safety in pigs of PHH-1V, a SARS-CoV-2 RBD fusion heterodimer vaccine candidate

This study evaluates the immunogenicity and safety of PHH-1V, a SARS-CoV-2 RBD fusion vaccine in pigs, showing a robust immune response and good safety profile.

Preclinical evaluation of PHH-1V vaccine candidate against SARS-CoV-2 in non-human primates

This study evaluates the PHH-1V vaccine candidate against SARS-CoV-2 in non-human primates, showing high antibody levels, balanced cellular immunity, and reduced viral load.

Characterization of p38a signaling networks in cancer cells using quantitative proteomics and phosphoproteomics

This study identifies p38-regulated proteins and phosphoproteins in breast cancer cells, revealing its role in cell adhesion, DNA replication, and RNA metabolism.

Lipid-mediated dimerization of membrane-anchored c-Src is driven by a cluster of lysine residues in the N-terminal SH4 domain.

This study identifies a cluster of lysine residues in the SH4 domain of c-Src that enhances lipid-mediated dimerization, increasing tyrosine phosphorylation in HEK293T cells.

Evolution of CRISPR-associated Endonucleases as Inferred from Resurrected Proteins

This study resurrects ancient CRISPR-Cas9 nucleases to show their greater flexibility in PAM and gRNA requirements and their evolution toward double-strand break activity.

Cetacean "gas-bubble thromboembolic polycystic liver disease": "Budd-Chiari-like syndrome" in dolphins?

The paper reinterprets cystic liver lesions in cetaceans as Budd-Chiari-like syndrome caused by gas bubbles and pre-existing vascular disorders, not acoustic trauma.

Mapping functional regions of essential bacterial proteins with dominant-negative protein fragments

This paper maps inhibitory activity of protein fragments against ten essential bacterial proteins, revealing principles of fragment-based inhibition and their relationship to protein interactions.

Preclinical evaluation of a COVID-19 vaccine candidate based on a recombinant RBD fusion heterodimer of SARS-CoV-2

This paper evaluates a recombinant RBD fusion heterodimer vaccine in mice, showing 100% efficacy against SARS-CoV-2 variants and neutralizing antibodies.

Mouse lemur, a new animal model to investigate cardiac pacemaker activity in primates.

This study characterizes cardiac pacemaker activity in mouse lemurs, revealing similarities to human SAN function and potential for modeling age-related cardiovascular dysfunctions.

LORE receptor homomerization is required for 3-hydroxydecanoic acid-induced immune signaling and determines the natural variation of immunosensitivity within the Arabidopsis genus

This study shows that LORE receptor homomerization is essential for 3-hydroxydecanoic acid-induced immune signaling and explains natural variation in immunosensitivity among Arabidopsis species.

Cell Communication Network factor 4 promotes tumor-induced immunosuppression in melanoma

This study shows that CCN4 promotes immunosuppression in melanoma by reducing CD8+ T cells and increasing MDSCs, and that its removal enhances immune checkpoint therapy effectiveness.

SUMOylation regulates protein cargo in Astrocyte-derived small extracellular vesicles

This study investigates how SUMOylation affects protein cargo in astrocyte-derived small extracellular vesicles and shows that it influences the physiological function of target cells.

Bradycardic mice undergo effective heart rate improvement after specific homing to the sino-atrial node and differentiation of adult muscle derived stem cells.

This study shows that adult muscle-derived stem cells can differentiate into cardiac pacemaker cells and improve heart rate in bradycardic mice.

Focused ultrasound stimulates ER localized mechanosensitive PANNEXIN-1 to mediate intracellular calcium release in invasive cancer cells

This study shows focused ultrasound activates ER-localized PANX1 in invasive cancer cells, triggering calcium release and cytokine release, suggesting a new adjuvant therapy for cancer immunotherapy.

Phenotypic functional activities of monocyte change during crosstalk with breast cancer cell and enhancing effect of metformin of IFN-γ-associated antitumor cytokine production.

This study examines how metformin affects monocyte function during interaction with breast cancer cells, showing increased IFN-γ production and reduced tumor cell proliferation.

Phenylethynylbenzyl-Modified Biguanides Inhibit Pancreatic Cancer Tumor Growth

The paper designs and tests phenylethynylbenzyl-modified biguanides, showing they inhibit pancreatic cancer growth more effectively than existing biguanides.

Cell Communication Network factor 4 (CCN4/WISP1) shifts melanoma cells from a fragile proliferative state to a resilient metastatic state

This study shows CCN4 promotes melanoma metastasis by shifting cells from a proliferative to a resilient state, with implications for therapy and biomarker development.

Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27

The paper shows Myc activates Cdk1 to phosphorylate p27 at Thr187, promoting its degradation and cell cycle progression, with Cdk1 inhibition causing synthetic lethality with Myc.

WNT1 Inducible Signaling Pathway Protein 1 (WISP1) Stimulates Melanoma Cell Invasion and Metastasis by Promoting Epithelial - Mesenchymal Transition (EMT)

This study shows WISP1 promotes melanoma invasion and metastasis by inducing EMT through Akt and MAP kinase pathways.

Highly differentiated CD4 T cells Unequivocally Identify Primary Resistance and Risk of Hyperprogression to PD-L1/PD-1 Immune Checkpoint Blockade in Lung Cancer

The paper identifies baseline circulating highly-differentiated CD4 T cells as predictors of response or hyperprogression to PD-L1/PD-1 blockade therapy in lung cancer patients.