Author

B. P. Kleinstiver

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Bioengineering· 1 Biophysics· 1 Evolutionary Biology· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:cell signaling & trafficking· 2 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Research Focus:protein folding & dynamics· 2 Research Focus:variant calling· 2 Assay:whole-genome sequencing· 2 Organism:bacteria (other)· 1

Frequent Co-Authors

K. A. Christie· 2 shared L. Ma· 2 shared A. Diaz· 1 shared A. Fernandez· 1 shared A. Quesada-Ganuza· 1 shared A. R. Davidson· 1 shared A. Reifs· 1 shared A. Sanchez-Mejias· 1 shared

Activity by Year

2017-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 14 mutual co-author links

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2 shared papersBiochemistry

K. A. Christie

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Author profile →

6 papers

Recent·Most cited

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases

J. F. da Silva, C. J. Tou, E. M. King, M. L. Eller, L. Ma, D. Rufino-Ramos, B. P. Kleinstiver

This paper introduces click editing, a genome writing platform that enables precise installation of genetic edits using DNA polymerases and HUH endonucleases, with minimal indels and no unwanted insertions.

bioRxiv · Sep 12, 2023 · PreprintBioengineering

In situ architecture of Opa1-dependent mitochondrial cristae remodeling

M. Y. Fry, P. P. Navarro, P. Hakim, V. Y. Ananda, X. Qin, J. C. Landoni, S. Rath, Z. Inde, C. M. Lugo, B. E. Luce, Y. Ge, J. L. McDonald, I. Ali, L. L. Ha, B. P. Kleinstiver, , L. H. Chao

This study uses cryo-electron tomography to visualize how different Opa1 forms affect mitochondrial cristae architecture and function in mouse cells.

bioRxiv · Jan 16, 2023 · PreprintBiophysics

Evolution of CRISPR-associated Endonucleases as Inferred from Resurrected Proteins

B. Alonso-Lerma, Y. Jabalera, M. Morin, A. Fernandez, S. Samperio, A. Quesada-Ganuza, A. Reifs, S. Fernandez-Penalver, Y. Benitez, L. Soletto, J. A. Gavira, A. Diaz, W. Vranken, B. P. Kleinstiver, A. Sanchez-Mejias, , R. Perez-Jimenez

This study resurrects ancient CRISPR-Cas9 nucleases to show their greater flexibility in PAM and gRNA requirements and their evolution toward double-strand break activity.

bioRxiv · Mar 30, 2022Evolutionary Biology

Precise DNA Cloning via PAMless CRISPR-SpRYgests

K. A. Christie, J. A. Guo, R. A. Silverstein, R. M. Doll, M. Mabuchi, H. E. Stutzman, L. Ma, G. B. Robb, B. P. Kleinstiver

This paper introduces SpRYgests, a CRISPR-based DNA cloning method that enables precise cleavage at any sequence, improving cloning workflows and reducing costs compared to restriction enzymes.

bioRxiv · Jan 11, 2022 · PreprintBiochemistry

Listeria phages induce Cas9 degradation to protect lysogenic genomes

B. A. Osuna, S. Karambelkar, C. Mahendra, K. A. Christie, B. Garcia, A. R. Davidson, B. P. Kleinstiver, S. Kilcher, J. Bondy-Denomy

This study identifies AcrIIA1 as a phage-encoded protein that inhibits Cas9 in Listeria monocytogenes by promoting its degradation, protecting lysogenic genomes.

bioRxiv · Sep 30, 2019Microbiology

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

J. S. Chen, Y. S. Dagdas, B. P. Kleinstiver, M. M. Welch, L. B. Harrington, S. H. Sternberg, J. K. Joung, A. Yildiz, J. A. Doudna

This study identifies a mechanism by which CRISPR-Cas9 variants discriminate target mismatches and introduces a hyper-accurate variant with improved specificity.

bioRxiv · Aug 12, 2017Biochemistry