Genome-wide CRISPR knockout screening with viral replicons for identification of host factors involved in viral replication
Karen W. Cheng, Madhura Bhave, Andrew L. Markhard, Duo Peng, Karan D. Bhatt, Katherine A. Travisano, Josette V. Medicielo, Sanae Lembirik, Leila Njoya, Manu Anantpadma, Jens H. Kuhn, Andreas S. Puschnik, Amy L. Kistler
This paper develops a CRISPR knockout screening method using viral replicons to identify host factors essential for viral replication, successfully identifying known and novel genes for dengue, chikungunya, and Ebola viruses.
bioRxiv · Jan 9, 2025 · PreprintGenomics