Enhanced in vivo gene knockout with undetectable off-targets using multiplexed Cas12a sgRNAs
Fillip Port, Martha A Buhmann, Jun Zhou, Mona Stricker, Alexander Vaughan-Brown, Ann-Christin Michalsen, Eva Rossmanith, Amélie Pöltl, Lena Grosskurth, Julia Huber, Laura B Menendez Kury, Bea Weberbauer, Maria Hübl, Florian Heigwer, Michael Boutros
The paper presents a Cas12a-based system using multiplexed sgRNAs to achieve efficient and specific gene knockouts in Drosophila with minimal off-target effects.
bioRxiv · Nov 26, 2024 · PreprintGenetics