Fillip Port

Proliferation and differentiation of intestinal stem cells depends on the zinc finger transcription factor BCL11/Chronophage

This study identifies Cph as a key regulator of intestinal stem cell proliferation and differentiation, showing its role in maintaining ISC and EE populations and remodeling progenitor transcriptomes.

Secretion and signaling properties of Wnt11 and Wnt3 are determined by their N-terminus

This study shows that the N-terminus of Wnt proteins determines their secretion and signaling properties, with Wnt11's N-terminus enabling Evi/Wls-independent secretion and Frizzled 6 activation.

Class III PI3K is essential for Wingless secretion and Evi/Wls recycling in Drosophila

The paper identifies Vps15 as essential for endocytosis-dependent Wg secretion and reveals divergent post-endocytic fates of Wg and Evi/Wls in Drosophila.

Enhanced in vivo gene knockout with undetectable off-targets using multiplexed Cas12a sgRNAs

The paper presents a Cas12a-based system using multiplexed sgRNAs to achieve efficient and specific gene knockouts in Drosophila with minimal off-target effects.

Restraining Wnt activation and intestinal tumorigenesis by a Rab35 dependent GTPase relay

This study identifies Rab35 as a tumor suppressor that modulates Wnt signaling through a GTPase relay, highlighting its role in restraining intestinal tumorigenesis.

The pleiotropic functions of Pri smORF peptides synchronise leg development regulators

The paper identifies Pri smORF peptides as pleiotropic regulators of leg development in Drosophila, functioning through Ubr3-dependent and -independent mechanisms in patterning and cell survival.

A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene inactivation in vivo

This paper develops a temperature-tolerant CRISPR base editor for efficient and precise gene inactivation in Drosophila, showing high efficiency and minimal byproducts.

Multiplexed conditional genome editing with Cas12a in Drosophila

This paper demonstrates efficient Cas12a-based gene editing in Drosophila, enabling temperature-controlled editing, multiplex targeting, and cell-type-specific manipulation.

Systematic functional analysis of Rab GTPasesreveals limits of neuronal robustness in Drosophila

This study systematically analyzes Rab GTPase function in Drosophila neurons, revealing that some Rabs are critical for robustness to environmental challenges despite being viable in null mutants.

A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila

The paper introduces a large-scale CRISPR mutagenesis library in Drosophila for targeted gene disruption, showing high efficiency and revealing new developmentally essential genes.