Florian Heigwer

The paper identifies regulators of Dvl2 biomolecular condensates using image-based screens and phosphoproteomics, finding CK1δ/ε inhibition blocks condensate formation and modulates Wnt signaling.

The paper presents a Cas12a-based system using multiplexed sgRNAs to achieve efficient and specific gene knockouts in Drosophila with minimal off-target effects.

This study shows that aged intestinal epithelial cells exhibit a persistent inflammation signature, marked by MHC class II upregulation and increased chromatin accessibility of inflammation-associated loci.

The paper introduces a large-scale CRISPR mutagenesis library in Drosophila for targeted gene disruption, showing high efficiency and revealing new developmentally essential genes.

The paper identifies lineage-specific core-regulatory circuits in cancer cells and shows their role in gene essentiality and therapeutic vulnerability.

The paper identifies time-dependent genetic interactions in Drosophila cells using RNAi screens and develops an algorithm to analyze rewiring of signaling pathways.

The paper develops MINGLE, a computational framework to integrate CRISPR/Cas9 screens and identify genetic interactions in cancer cells, revealing new vulnerabilities and building a large genetic network.

HTSvis is a web app for interactive visualization and exploratory analysis of arrayed high-throughput screens, supporting both cellHTS and generic data tables.

CRISPRAnalyzeR is a web application that unifies eight hit-calling methods and 26 external annotation resources to analyze, visualize, and document pooled CRISPR/Cas9 screens without requiring bioinformatics expertise.

This paper develops a method to phenotype human induced pluripotent stem cells using high-throughput imaging, identifying key regulators of pluripotency that are masked in population-averaged analyses.