Jun Zhou

The paper presents a Cas12a-based system using multiplexed sgRNAs to achieve efficient and specific gene knockouts in Drosophila with minimal off-target effects.

This study shows that JNK signaling disruption in intestinal tumors leads to barrier failure and microbiome imbalance, which can be reversed by JNK inhibition or microbiome depletion.

The paper introduces a large-scale CRISPR mutagenesis library in Drosophila for targeted gene disruption, showing high efficiency and revealing new developmentally essential genes.

The paper identifies key regulatory mechanisms in Drosophila male stem cells and provides a public dataset and tool for analyzing stem cell gene networks.