Analysis of Essential Genes in Clostridioides difficile by CRISPRi and Tn-seq
Maia E. Alberts, Micaila P. Kurtz, Ute Müh, Jonathon P. Bernardi, Kevin W. Bollinger, Horia A. Dobrila, Leonard Duncan, Hannah M. Laster, Andres J. Orea, Anthony G. Pannullo, Juan G. Rivera-Rosado, Facundo V. Torres, Craig D. Ellermeier, David S. Weiss
This study identifies essential genes in C. difficile using CRISPRi and Tn-seq, confirming most previously reported genes and identifying new ones, with comparisons to B. subtilis.
Journal of Bacteriology · Sep 8, 2025Peer-reviewedMicrobiology