Author

Kevin W. Bollinger

University of IowaORCID 0000-0002-7694-2200Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Organism:C. elegans· 2 Research Focus:cell type annotation· 2 Approach:genetic perturbation / CRISPR· 2 Assay:proteomics· 2 Organism:bacteria (other)· 1 Approach:biophysical modeling· 1 Research Focus:gene regulatory network inference· 1 Assay:metagenomics· 1

Frequent Co-Authors

Craig D. EllermeieriD· 3 shared David S. WeissiD· 3 shared Ute MühiD· 3 shared David L. PophamiD· 2 shared Alexis J. Apostolos· 1 shared Andres J. Orea· 1 shared Anthony G. Pannullo· 1 shared Facundo V. Torres· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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3 shared papersMicrobiology

Craig D. EllermeierORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

Carboxypeptidase activity drives L,D-transpeptidase essentiality during vegetative growth and sporulation in Clostridioides difficile

Kevin W. Bollinger, Ute Müh, Paige B Brannen, David L. Popham, David S. Weiss, Craig D. Ellermeier

This paper identifies DD-CPases as the source of tetrapeptides for LDTs in C. difficile and shows that LDTs are essential due to high DD-CPase activity, not for divisome/elongasome function.

mBio · Oct 6, 2026Peer-reviewedMicrobiology

Analysis of Essential Genes in Clostridioides difficile by CRISPRi and Tn-seq

Maia E. Alberts, Micaila P. Kurtz, Ute Müh, Jonathon P. Bernardi, Kevin W. Bollinger, Horia A. Dobrila, Leonard Duncan, Hannah M. Laster, Andres J. Orea, Anthony G. Pannullo, Juan G. Rivera-Rosado, Facundo V. Torres, Craig D. Ellermeier, David S. Weiss

This study identifies essential genes in C. difficile using CRISPRi and Tn-seq, confirming most previously reported genes and identifying new ones, with comparisons to B. subtilis.

Journal of Bacteriology · Sep 8, 2025Peer-reviewedMicrobiology

Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in Clostridioides difficile

Kevin W. Bollinger, Ute Müh, Karl L. Ocius, Alexis J. Apostolos, Marcos M. Pires, Richard F. Helm, David L. Popham, David S. Weiss, Craig D. Ellermeier

This study identifies a new family of LDTs in C. difficile that catalyze 3-3 peptidoglycan crosslinking, essential for viability and distinct from previously known YkuD domain LDTs.

Proceedings of the National Academy of Sciences · Aug 16, 2024Peer-reviewedMicrobiology