Micaila P. Kurtz

This paper identifies CenIR as an essential BlaIR-family regulatory system in C. difficile that lacks a β-lactam-binding domain and is required for viability, with its regulon containing genes involved in peptidoglycan hydrolase and cell envelope maintenance.

This study identifies essential genes in C. difficile using CRISPRi and Tn-seq, confirming most previously reported genes and identifying new ones, with comparisons to B. subtilis.